Chaos in the diagnosis of hepatitis C virus inhemodialysis patients
Main Article Content
Keywords
epatitis C virus; Hemodialysis; Antibody detection; Molecular method
Abstract
Background: In India, a broad range of prevalence of Hepatitis C virus (HCV) infection in hemodialysis (HD) patients was reported. Variations in screening methods and regional differences in the HCV genome render challenges in the accurate detection of HCV. In this background, the present study was undertaken to assess the prevalence of HCV by HCV RNA PCR (Ribose nucleic acid Polymerase Chain Reaction) and HCV antibody detection by immunochromatography (ICT) and ELISA (Enzyme Linked Immuno-Sorbent Assay). Methods: A total of 50 patients with chronic kidney disease (CKD) on maintenance HD for more than six months were included in the study. Blood sample was collected and subjected to HCV RNA PCR, and HCV antibody by ICT and ELISA. Results: Of the 50 samples tested, ICT for HCV antibody was detected in 22 (44%) patients. They were positive for ELISA also. HCV RNA was positive in 15 (30%) patients. PCR positive was also positive for HCV antibody. Conclusions: This study concludes, that HCV antibody screening should be performed monthly and RNA PCR once every three months among HD patients.
References
[2] Le DHH, Kanokudom S, Nguyen HM, Yorsaeng R, Honsawek S, Vongpunsawad S, et al. Hepatitis C virus—core antigen implications in diagnostic, treatment monitoring and clinical outcomes. Viruses. 2024; 16: 1863.
[3] Anand A, Shalimar. Hepatitis C virus in India: challenges and successes. Clinical Liver Disease. 2021; 18: 150–154.
[4] Bao K, Chen J, Liu R, Xiang Y, Gao W. Prevalence of HCV infection among hemodialysis patients in Lanzhou of Northwestern China. Infection and Drug Resistance. 2022; 15: 5609–5617.
[5] Altinawe J, Eid Akkawi M, Kharrat Helu N, Hassan Q, Nattouf AH. Seroprevalence and risk factors of HBV, HCV and HIV among hemodialysis patients a multicenter cross-sectional study from Damascus Syria. BMC Infectious Diseases. 2024; 24: 289.
[6] Rajasekaran C, Kalpanaraj D, Banu ST, Duraivel M. Seroprevalence of hepatitis C virus infection among hemodialysis patients in a tertiary care hospital in South India. Journal of Pure and Applied Microbiology. 2023; 17: 371–379.
[7] Dharmesti NWW, Wibawa IDN, Kandarini Y. Hepatitis C seroconversion remains high among patients with regular hemodialysis: study of associated risk factors. International Journal of Hepatology. 2022; 2022: 8109977.
[8] Austria A, Wu GY. Occult hepatitis C virus infection: a review. Journal of Clinical and Translational Hepatology. 2018; 6: 155–160.
[9] Madhavan A, Sachu A, Balakrishnan AK, Vasudevan A, Balakrishnan S, Vasudevapanicker J. Prevalence of hepatitis C among hemodialysis patients in a tertiary care hospital in South India. Iranian Journal of Microbiology. 2020; 12: 644–649.
[10] Reddy AK, Murthy KVD, Lakshmi V. Prevalence of HCV infection in patients on hemodialysis: survey by antibody and core antigen detection. Indian Journal of Medical Microbiology. 2005; 23: 106–110.
[11] Medhi S, Potukuchi SK, Polipalli SK, Swargiary SS, Deka P, Chaudhary A, et al. Diagnostic utility of hepatitis C virus core antigen in hemodialysis patients. Clinical Biochemistry. 2008; 41: 447–452.
[12] Kerollos KMN, El-Ameen HA, El Wahed LA, Azoz NMA. Prevalence and seroconversion of hepatitis C among hemodialysis patients in Assiut governorate, Egypt. The Egyptian Journal of Internal Medicine. 2020; 32: 2.
[13] Méndez-Sánchez N, Motola-Kuba D, Chavez-Tapia NC, Bahena J, Correa-Rotter R, Uribe M. Prevalence of hepatitis C virus infection among hemodialysis patients at a tertiary-care hospital in Mexico City, Mexico. Journal of Clinical Microbiology. 2004; 42: 4321–4322.
[14] Alter MJ, Kuhnert WL, Finelli L; Centers for Disease Control and Prevention. Guidelines for laboratory testing and result reporting of antibody to hepatitis C virus. Centers for Disease Control and Prevention. MMWR. Recommendations and Reports. 2003; 52: 1–13, 15; quiz CE1–4.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Ananthapadmanabasamy Indira, Department of Microbiology & Virology, Tirunelveli Medical College, 627011 Tirunelveli, India
Senior Resident, Department of Virology
Gomathinayagam Velvizhi, Department of Microbiology & Virology, Tirunelveli Medical College, 627011 Tirunelveli, India
Senior Resident, Department of Virology
Koshy M Koshy, Department of Microbiology & Virology, Tirunelveli Medical College, 627011 Tirunelveli, India
Senior Assistant Professor, Department of Microbiology
Santhana Kumarasamy Poongodi, Department of Microbiology & Virology, Tirunelveli Medical College, 627011 Tirunelveli, India
1